Quantifying terpenes in rumen fluid, serum, and plasma from sheep

TitleQuantifying terpenes in rumen fluid, serum, and plasma from sheep
Publication TypeConference Paper
Year of Publication2010
AuthorsEstell RE, Utsumi S.A, Cibils AF
Conference NameJournal of Animal Science
ARIS Log Number255805
Keywordsdaily gain, mesquite, steers
AbstractDetermining the fate of terpenes consumed by browsing ruminants require methods to quantify their presence in blood and rumen fluid. Our objective was to modify an existing procedure for plasma terpenes to quantify 25 structurally diverse mono- and sesquiterpenes in serum, plasma, and rumen fluid from sheep. The terpenes examined were tricyclene, α-pinene, camphene, sabinene, β-pinene, myrcene, 2-carene, 3-carene, α-terpinene, p-cymene, limonene, 1,8-cineole, cis-β-ocimene, γ-terpinene, cis-sabinene hydrate, terpinolene, linalool, camphor, borneol, terpin-4-ol, α-terpineol, longifolene, β-caryophyllene, α-humulene, and caryophyllene oxide. Terpenes were extracted with SPE columns and quantified using gas chromatography (n = 8 per terpene/fluid combination). Data were analyzed with the MIXED procedure of SAS with fluid as the independent factor, and means were separated by LSD in the event of a significant F test (α = 0.05). Recovery estimates were 100± 5% for 14, 7, and 4 terpenes from serum, plasma, and rumen fluid, respectively. Recovery from plasma and serum differed for 12 terpenes (P < 0.05), although typically differences were <10%. Recovery from rumen fluid differed (P < 0.05) from both serum and plasma for 16 compounds (lower in each case except linalool). Recovery did not differ (P > 0.05) among the 3 matrices for only 2 compounds (p-cymene and terpinolene). Greater recovery was generally observed for oxygenated terpenes than hydrocarbon compounds, particularly for monoterpenes. This procedure is applicable to a wide array of terpenes in fluids from sheep, but differential recoveries among terpenes and fluids require that estimated concentrations of each analyte be corrected for recovery using that specific compound in the same matrix collected under the same set of experimental conditions, and that caution be exercised in generalizing responses among different compounds with this procedure.