Application of Emission-Excitation Fluorescence Spectrum for the Determination of Plants in Goat Diets

Title	Application of Emission-Excitation Fluorescence Spectrum for the Determination of Plants in Goat Diets
Publication Type	Conference Paper
Year of Publication	2005
Authors	Pezzotti C., Sisk C.N., Obeidat S., Rayson G.D., Anderson D.M.
Conference Name	Pittsburg Conference on Analytical Chemistry and Applied Spectroscopy
Date Published	February 26-Marc
Conference Location	Orlando, FL
ARIS Log Number	179327
Keywords	diets, emission-excitation, goats
Abstract	The use of goats for the selective control of invasive shrub in grassland is environmentally desirable. To amplify any genetically based preferences for specific plant species it is necessary to determine the presence of targeted plants in the animals’ diets. Currently there are two proposed techniques for analysis of composition of post- and pre- digested diets, near infrared reflectance spectroscopy (NIRS) and fluorescence spectroscopy. Although NIRS has been successfully used to determine the botanical composition of animal feed, it is limited to the availability of training samples that closely match the animals’ diets. We have proposed the use of multidimensional fluorescence measurements as a detection technique that may be less-sensitive to animal diet variations. Fluorescence intensity response surfaces as functions of both excitation and emission wavelengths have been generated for buffered saline extracts of both feed and fecal samples from a study directed toward the identification of juniper species within goat diets. Classical chemometric techniques have been applied to these data that include multi-way principle components analysis (MPCA) and parallel factor analysis (PARAFAC). The results of these studies will be presented. The implications of those results to the use of fluorescence spectroscopy for the identification of plants in goat diets will be discussed.