@conference {50713,
	title = {In vitro mass clonal propagation and hyperhydricity reversal of four-wing saltbush (<i>Atriplex canescens</i> (Pursh) Nutt.)},
	booktitle = {2004 World Congress on In Vitro Biology},
	year = {2004},
	month = {May 2004},
	address = {San Francisco, CA},
	abstract = {A successful, in vitro mass propagation system for cloning four-wing saltbush, Atriplex canescens (Pursh) Nutt., was achieved.  In vitro propagated shoots of A. canescens showed severe symptoms of hyperhydricity, a frequent physiological problem reported in miro-propagation of A. canescens and other plant species.  Hyperhydricity was exacerbated when cultured in airtight culture vessels and high ammonium concentraqtions in common media formulations and drastically reduced the quality of in vitro cultures.  Hyperhydric shoots reverted to normal shoots by culturing on modified Murashige and Skoog basal medium and using larger culture vessels with vented closures.  Resulting normal shoots significantly improved all aspects of micro-propagation.  Shoots were rooted in vitro by supplementing the culture medium with 2.45 uM of indolebutyric acid (IBA).  Plants were successfully reestablished in soil transplanted and surviving in native sites.},
	author = {Reyes, I. and Hubstenberger, J. and J.R. Barrow}
}
